Attempts to obtain miracidia

Eggs were recovered from gravid adult specimens of Bucephalus longicornutus that had been established experimentally in Scorpaena cardinalis. The eggs were divided into two samples and placed in separate Syracuse watch glasses, one of which contained sea-water and the other, sea-water diluted by two and a-half times page 6 its volume with distilled water to make it isosmotic with fish body fluids. The latter is termed diluted sea-water in the remainder of the text. It was unknown whether the eggs might hatch in sea-water or in the rectum of the host and therefore the two possibilities were considered for this experiment. The eggs were examined daily.